Antigen

Antigen Class

TFs and others

Antigen

Nfia

Cell type

Cell type Class

Neural

Cell type

Olfactory Bulb

MeSH Description

Ovoid body resting on the CRIBRIFORM PLATE of the ethmoid bone where the OLFACTORY NERVE terminates. The olfactory bulb contains several types of nerve cells including the mitral cells, on whose DENDRITES the olfactory nerve synapses, forming the olfactory glomeruli. The accessory olfactory bulb, which receives the projection from the VOMERONASAL ORGAN via the vomeronasal nerve, is also included here.

Sample

source_name

Olfactory bulb

strain

C57BL6

tissue

Olfactory bulb

age

20-weeks

chip antibody

NFIA

transfection

siRNA-scramble

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates from 20-week old wild type hippocampus and olfactory bulb were sonicated, and NFIA-DNA complexes were purified with antibody in triplicates. NFIA-DNA complexes were isolated from siRNA-NFIB and siRNA-scramble olfactory bulb in duplicate. DNA libraries were constructed from 5-10ng of chromatin immunoprecipitated DNA using the TruSeq ChIP library preparation kit (Cat# IP-202-1012) following the manufactures instructions. Libraries were validated on a fragment analyzer to verify successful gel extraction.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

32020849

Reads aligned (%)

95.8

Duplicates removed (%)

21.7

Number of peaks

330 (qval < 1E-05)

mm9

Number of total reads

32020849

Reads aligned (%)

95.6

Duplicates removed (%)

21.7

Number of peaks

307 (qval < 1E-05)